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Research Article |

Study on Prp-Induced Chondrogenesis of Adipose Stem Cells

Background: In the field of repair and reconstruction surgery, the use of cartilage is large, but the source of cartilage is small; adipose stem cells come from a wide range of sources and can be induced to differentiate into a variety of cells under specific circumstances. By using platelet-rich plasma to induce differentiation of adipose stem cells into chondrocytes, tissue engineering construction of cartilage will become possible. Objective: The optimal culture conditions for PRP-induced differentiation of adipose stem cells into chondrocytes were screened, and REAL-TIME PCR detection of aggrecan and sox-9 were used to confirm that adipose stem cells can be induced by PRP to differentiate into chondrocytes. Methods: After isolating and culturing adipose stem cells, use DMEM culture medium containing 0, 5% and 10% PRP to subculture the cells to induce differentiation. The CCK8 kit detects cell proliferation; use DMEM culture medium containing 5% PRP to culture adipose stem cells. Cells were collected on days 3, 7, 14 and 21 of culture, and Real-Time PCR were performed to detect specific antigens aggrecan and sox-9. Results: In the DMEM culture medium containing 5% PRP, adipose stem cells grow well and are not prone to aggregation reactions. On the 3rd, 7th, 14th and 21st days of differentiation induced by 5% PRP, Real-Time PCR tests found that aggrecan and sox-9 were continuously expressed, and their expression was found to be progressively enhanced. Conclusion: DMEM medium containing 5% PRP is a suitable medium for inducing the differentiation of adipose stem cells into chondrocytes. Adipose stem cells can be used as seed cells to differentiate into chondrocytes after being induced by PRP and have the characteristic antigen expression of chondrocytes.

Platelet-Rich Plasma (Prp), Adipose Stem Cells (Adsc), Chondrocytes, Aggrecan, Sox-9

APA Style

Lun Liu, Bin Yang, Bin Xu. (2023). Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Advances in Surgical Sciences, 11(2), 32-35. https://doi.org/10.11648/j.ass.20231102.13

ACS Style

Lun Liu; Bin Yang; Bin Xu. Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Adv. Surg. Sci. 2023, 11(2), 32-35. doi: 10.11648/j.ass.20231102.13

AMA Style

Lun Liu, Bin Yang, Bin Xu. Study on Prp-Induced Chondrogenesis of Adipose Stem Cells. Adv Surg Sci. 2023;11(2):32-35. doi: 10.11648/j.ass.20231102.13

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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